Neeti Mohan was born in Delhi. Her father, Brij Mohan Sharma, is a government officer and her mother, Kusum, is a homemaker. Mohan is the eldest of four sisters; Shakti Mohan, Mukti Mohan and Kriti Mohan. Mohan initiated in learning music at the Gandharva Mahavidyalaya. During the time, she was involved in dramatics and dancing; considering singing as a "serious hobby". She later moved to Birla Balika Vidyapeeth, Pilani, where she heeded the music and songs from Lata Mangeshkar and Asha Bhosle. "I would keep playing the tapes and try to sing like Ashaji. No one was really there to correct me. It was mostly about self-correction".
During her school days, Mohan was part of the school band, and participated in Delhi Republic Day parade for five years in a row. Convinced by her father, Mohan participated in many extra-curricular activities during her school period; singing, dancing, performing in the band and theatre. She was adjudged best cadet in the National Cadet Corps of India and represented India as Youth Exchange Programme cadet. Later, she learnt music formally at the Bhatkhande Music Institute and continued training in Mumbai with Rajashree Pathak, for five years. Apart from training in singing, Mohan studied dancing with her two sisters. She trained in Bharata Natyam and Kathak under the supervision of Ashley Lobo. Besides training kids to dance, Mohan worked as an instructor there. Mohan graduated in philosophy from Miranda House, University of Delhi.
In 2003, Mohan was one of the winners of the Channel V's reality television show Popstars, subsequently being chosen for Aasma—a pop group formed with the other winners of the show where she got the chance to perform along with Bryan Adams. Insisted by Deepak Gattani, who manages A.R. Rahman's concert tours, Mohan managed to catch the attention of Rahman when she randomly sent a demo CD to him and was offered a chance to join his musical troupe. While she was assisting Lobo, he proffered Mohan to be featured in a supporting role in Imtiaz Ali-directed film, Socha Na Tha (2005), while Lobo was working as the choreographer in the film. In 2009, Mohan participated in the Star Plus show Music Ka Maha Muqqabla, where she was part of the winning team "Shankar's Rockstars" led by Shankar Mahadevan.